Deleted
Experience: None
Not Used
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Plasmid_Backbone

Part:BBa_K209105:Design

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-10-16)


pDONR221


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2288
    Illegal PstI site found at 1163
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2288
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal PstI site found at 1163
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2288
    Illegal BamHI site found at 1832
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2288
    Illegal PstI site found at 1163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2288
    Illegal PstI site found at 1163
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1282
    Illegal SapI.rc site found at 116


Design Notes

Source

References